Potential application of FoldX force field based protein modeling in zinc finger nucleases design
نویسندگان
چکیده
منابع مشابه
Genome engineering with zinc-finger nucleases.
Zinc-finger nucleases (ZFNs) are targetable DNA cleavage reagents that have been adopted as gene-targeting tools. ZFN-induced double-strand breaks are subject to cellular DNA repair processes that lead to both targeted mutagenesis and targeted gene replacement at remarkably high frequencies. This article briefly reviews the history of ZFN development and summarizes applications that have been m...
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Zinc finger nucleases (ZFNs) facilitate tailor-made genomic modifications in vivo through the creation of targeted double-stranded breaks. They have been employed to modify the genomes of plants and animals, and cell-based therapies utilizing ZFNs are undergoing clinical trials. However, many ZFNs display dose-dependent toxicity presumably due to the generation of undesired double-stranded brea...
متن کاملDesign and Development of Artificial Zinc Finger Transcription Factors and Zinc Finger Nucleases to the hTERT Locus
The ability to direct human telomerase reverse transcriptase (hTERT) expression through either genetic control or tunable regulatory factors would advance not only our understanding of the transcriptional regulation of this gene, but also potentially produce new strategies for addressing telomerase-associated disease. In this work, we describe the engineering of artificial zinc finger transcrip...
متن کاملPrecise Genome Modification in Maize Using Zinc-Finger Nucleases
Agricultural biotechnology is limited by the inefficiency and unpredictability of conventional random mutagenesis and transgenesis. We have developed a broadly applicable, versatile alternative: the use of designed zinc-finger nucleases (ZFNs) that induce double-stranded breaks (DSB) at their target locus. We show that ZFNs designed to cleave an endogenous maize gene disrupt their target via DS...
متن کاملEmerging gene knockout technology in zebrafish: zinc-finger nucleases.
One advantage of the zebrafish model system is the ability to use forward genetics to reveal critical gene functions by their mutant phenotype. Reverse genetic tools are available, although it is more challenging and time-consuming to identify mutations in specific genes of interest and virtually impossible to induce mutations in a targeted manner. Two recent papers have shown that locus-specif...
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ژورنال
عنوان ژورنال: Science China Life Sciences
سال: 2011
ISSN: 1674-7305,1869-1889
DOI: 10.1007/s11427-011-4159-9